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D. Platukytė professional bachelor thesis ,,Research of flavonoids and phenolic compounds in peppermint and spearmint leaves that grow in Lithuanian climate zone“. Supervisor lect. Aušra Adomėnienė; University of Applied Sciences, Faculty of Medicine, Departament of Pharmacy Technician – Kaunas, 2019. Keywords: peppermint, spearmint, phenolic compunds, flavonoids, spectrophotometry. The aim of this research: to examine total flavonoids and phenolic compounds in peppermint and spearmint leaves that grow in Lithuanian climate zone. The objectives of the Study: 1. Theoretically describe information about peppermint (Mentha piperita L.) and spearmint (Mentha spicata L.) medicinal plant raw material chemical composition, pharmacological effects and medical use. 2. To reveal the medical value og flavonoids and phenolic compounds. 3. Identify the identity of a medicinal plant raw material by a microscopic method. 4. To determine total phenolic and flavonoid content in peppermint (Mentha piperita L.) and spearmint (Mentha spicata L.) leaves. Methods: The 10 g dried plant material was extracted in 100 ml of distilled water at low heat (50 C) in a water bath and after every 2 hours the extract was filtered through a paper filter. The process was repeated 3 times. Total phenolic contents of peppermint (Mentha piperita L.) and spearmint (Mentha spicata L.) leaves extracts were estimated with spectrophotometry method, Folin – Ciocalteu reagent. The total phenolic contents were calculated based on the calibration curve of gallic acid mg/g. The total flavonoid content of the extracts fractions was estimated by aluminum (III) chloride reagent, flavonoid content was estimated in percent. Results and conclusion: The largest phenolic content was discovered in peppermint (Mentha piperita L.) leaves collected in Mazeikiai district (GAE 56,9 mg/g), while the smallest content was found inside peppermint collected in Kelme district (GAE 37,9 mg/g). Comparing the total phenolic content of spearmint (Mentha spicata L.) and peppermint (Mentha piperita L.) plant material, it was discovered that spearmint has more phenolic compound content than peppermint collected in Kelme district (GAE 43,4 mg/g). The largest flavanoid content were found inside peppermint collected in Kelme district (5,67 %), a little less was found in spearmint collected in Kaunas district (4,77 %), and the least was discovered inside peppermint collected in Mazeikiai district (2,76 %). After the microscopic analysis of the leaves of peppermint (Mentha piperita L.) and spearmint (Mentha spicata L.), it was found that the peppermint has larger glands of essential oil, but less often than the micropreparation of spearmint leaves. |
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Baigiamajame bakalauro darbe atskleista pipirmėtės ir šaltmėtės cheminė sudėtis, farmakologinis poveikis, taip pat aptarta flavonoidų ir fenolinių junginių reikšmė medicinoje.
Nustatytas bendras fenolinių junginių ir flavonoidų kiekis pipirmėtės ir šaltmėtės lapuose. Išnagrinėjus mokslinę literatūrą bei atlikus tyrimo duomenų analizę buvo pateiktos išvados. Darbą sudaro du skyriai pirmą skyrių sudaro literatūros analizė, kurios tikslas aprašyti pipirmėtės ir šaltmėtės cheminę sudėtį, farmakologinį poveikį ir medicininį panaudojimą. Antrame skyriuje aprašoma empirinio tyrimo metodika, pateikiami tyrimo rezultatai, kurio tikslas nustatyti fenolinių junginių kiekius esančius pipirmėtės ir šaltmėtės vaistinėje augalinėje žaliavoje - lapuose. Darbą sudaro: 13 paveikslėlių. Darbo apimtis: 34 puslapiai. 34 bibliografiniai šaltiniai |
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