Antiseptinių rankų priemonių antimikrobinis poveikis

Vereškaitė, Gabrielė

Dspace pagrindinis
→
Studentų baigiamieji darbai - Studijų programos
→
biomedicinos diagnostika
→
Peržiūrėti elementą

dc.contributor.author	Vereškaitė, Gabrielė
dc.date.accessioned	2019-06-20T08:21:39Z
dc.date.available	2019-06-20T08:21:39Z
dc.date.issued	2019-06-14
dc.identifier.uri	http://dspace.kaunokolegija.lt//handle/123456789/1643
dc.description	Disinfection is one of the main ways for preventing pollution and infectious diseases. Specialists of the Infectious Diseases and AIDS Center (ULAC) (2012), claim that “personal hygiene plays an important role in every human being life and helps to prevent many infectious diseases”. As dirty hands cause about 80 % of all infectious diseases, proper hand hygiene can reduce the spread of pathogenic microorganisms. For the prevention of infectious diseases, it is essential to follow the basic rules of hand hygiene. Objective: To evaluate the effect of hand antiseptics. Tasks: 1. To identify microbial sources of contamination, composition of antiseptics and the importance of hygiene in the prevention of infectious diseases. 2. To determine the effect of hand antiseptics with the help of qualitative microbiological research. 3. To evaluate the effectiveness of different hand antiseptics. It was taken 12 types of hand ethanol-based antiseptics of different manufacturers for the research. All of them were bought at pharmacies and supermarkets. Eight cultures of microorganisms were derived from OXOID no. 605068. The study was carried out by Muller-Hinton agar, a swab sputtering technique. The suspensions of microorganisms were produced according to McFarland's standard indicator to 0.5. Different microbial suspensions with sterile loops were placed in ninety-seven petri dishes on Muller-Hinton nutritional agar. Then the swabs soaked in 20 µl different hand antiseptics. Each swab with a sterile tweezer was spiked into nutritional medium with standard cultures (Staphylococcus aureus ATCC 29213, Staphylococcus epidermidis ATCC 12228, Enterococcus faecalis ATCC 29212, Esherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Proteus vulgaris ATCC 8427, Proteus mirabilis ATCC 6633, Candida albicans ATCC 10231). Petri dishes were incubated at + 36 ° C for 24 hours. After the incubation period, microbocidal and microbostatic effect of antiseptics around the swab was evaluated. The study was repeated three times. The sample 292 tests. In each study, biological control of microorganisms and Muller-Hinton's agar sterility control were performed. The researched proved that the strongest effect on microorganisms was provided by the hand antiseptic No.12. It was effective to all microorganisms. The weakest effect was by the hand antiseptic No. 4. It had no effect on germs of S.aureus, E. fecalis, E.coli and P.vulgaris	en_US
dc.description.abstract	Baigiamajame darbe nagrinėjama antiseptinių rankų priemonių antimikrobinis poveikis. Išnagrinėta mokslinė ir dalykinė literatūra apie rankų mikrobinės taršos šaltinius, antiseptinių priemonių sudėtį ir rankų higienos svarbą infekcinių ligų prevencijoje. Parengtos rezultatų išvados ir rekomendacijos. Darbas sudarytas iš įvado, literatūrinės dalies, empirinės tyrimo metodikos, rezultatų analizės, išvadų, rekomendacijų, literatūros sąrašo ir priedų. Darbo apimtis 38 psl. 33 literatūros šaltinių, 3 lentelių ir 11 paveikslų. Atskirai pridedami priedai	en_US
dc.language.iso	other	en_US
dc.subject	Antiseptinės rankų priemonės. Mikrobocidinis bei mikrobostatinis veikimas. Etaloninės mikroorganizmų kultūros. Rankų higiena. Hospitalinės infekcijos. Antiseptika. Rankų mikrobinė tarša	en_US
dc.title	Antiseptinių rankų priemonių antimikrobinis poveikis	en_US
dc.title.alternative	Antimicrobial Effect of Antiseptic Hand Hygiene Products	en_US
dc.type	Other	en_US