Medaus botaninės kilmės ir antimikrobinio poveikio vertinimas

Šaltys, Gediminas

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Medaus botaninės kilmės ir antimikrobinio poveikio vertinimas

Šaltys, Gediminas

URI: http://dspace.kaunokolegija.lt//handle/123456789/550

Data: 2018-06

Santrauka:

Didėjant mikroorganizmų atsparumui antibiotikams ir dezinfekuojančioms medžiagoms, mokslininkai ieško naujų preparatų, kurie veiktų bakteriocidiškai. Didelis dėmesys skiriamas natūralioms liaudies medicinoje vartojamoms vaistažolėms, prieskoniams, medui.

Description:

The aim of the study: To determine the relationship between the botanical origin of the honey and the antimicrobial effect. Objectives: 1. To analyse scientific literature on the main characteristics of honey; 2. To Determine the botanical origin of honey samples; 3. To evaluate whether the botanical origin of honey has antimicrobial effect on honey, based on the results of the research. There are know a lot of facts about the effect of honey to human health, such as, curing wounds, using when people catch cold, its good for your skin and ect. so it was used in the ancient medicine and the treatment of bee products is rapidly growing topic among scientists and physicians. Based on honey analysis methods, approved by the Minister of Agriculture (2000.01.10 Nr. 5 „Honey analysis methods“, determination of botanical origin is performed by microscopic preparation of honey smears. The work was carried out at the Kaunas College of Microbiology Laboratory. 33 samples have been chosen and it was found that 16 samples were rape honey, four samples monofloral - Sinapis arvensis. 1 sample is Lucerne (Medicago) honey. 2 samples - buckwheat (lot. Fagopyrum esculentum) honey. 6 samples - polifloral. 1 sample of strawberry linden (lot. Tilia). 1 sample - honey (lot. Calluna vulgaris). There are also 1 samples - Esparcets (lot. Onobrychis) and 1 sample of Thyme. Another research step was to investigate the antimicrobial effect of honey using a "well" to a rigid nutrient agar method. The antimicrobial effect was determined by measuring the diameter of the inhibition zone in millimeters. Nine reference cultures of the microorganisms were used for the study: Klebsiella Pneumoniae ATCC 31488, Pseudomona Aeruginosa ATCC 27853, Proteus Vulgaris ATCC 8427, Staphylococcus Aureus ATCC 25923, Enterococcus Faecalis ATCC 29212, Candida Albicans ATCC 10231, Staphylococcus Epidermidis ATCC 12228, Bacillus Cereus ATC 11778, Escherichia Coli ATCC 25922. It was found that, according to the botanical origin of the highest antimicrobial effect was noted thymus honey, it has antimicrobial effect on 8 cultures, and the lowest antimicrobial effect was alfalfa honey, which effected only one bacterial culture.

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