Santrauka:
Baigiamojo darbo tikslas - nustatyti skirtingos sudėties kontaktinių lęšių skysčių antimikrobinį veikimą skirtinguose jų poveikio į etalonines bakterijų kultūras laiko intervaluose. Baigiamajame darbe aptarta mokslinėje ir dalykinėje literatūroje pateikta informacija apie: ragenos ir junginės sandara, kontaktinių lęšių skysčių funkcijas, antimikrobines medžiagas, mikroorganizmus, sukeliančius akių infekcijas ir dažniausiai sukeliančių bakterijų akies ligų pasireiškimo požymius. Atliktas kokybinis mikrobiologinis tyrimas su skirtingų sudėčių kontaktinių lęšių skysčiais. Nustatyta, ar skiriasi skirtingos sudėties kontaktinių lęšių skysčių antimikrobinės savybės skirtingais poveikio į etaloninės bakterijų kultūras laiko intervalais (0,5 val., 1 val., 2 val., 4 val., 6 val. ir 8 val.), skaičiuojant gyvybingų bakterijų skaičių Miuler – Hintono agare. Darbo apimtis 39 psl., 6 iliustracijos, 1 lentelė, 34 bibliografiniai šaltiniai. Darbą sudaro įvadas, teorinė dalis, praktinė dalis, išvados, rekomendacijos, literatūros sąrašas ir priedai.
Description:
Relevance: Increasing popularity of contact lenses, which are placed on the cornea, are the perfect medium for bacterial eye infection if the person is not following the recommendations or hygiene requirements. An worldwide investigation was carried out, which showed that one third of the patients were new to wearing contact lenses, while majority of the patients (89 percent) were wearing them for four or more times a week (Morgan et al., 2018). Growing number of contact lenses users and using frequency increases the risk of eye infections. Contact lenses solutions are used to rinse, clearing filth, plaque, disinfect and moisturizing the lenses. Recommended upkeep of contact lenses depends on the composition of the solution. Objective of the research: determine the different contact lenses solutions antimicrobial effectiveness in correspondence to time. Tasks of the research: 1) To discuss the information found in scientific and subject-related literature about contact lenses solutions antimicrobial properties. 2) To compare the antimicrobial properties of different contact lenses solutions. 3) To research how the antimicrobial properties of contact lenses solutions changes over time. Methodology: The scientific and subject-related literature analysis about the antimicrobial properties of contact lenses solutions. A qualitative microbiological research was carried out with different contact lenses solutions. During the research selected standard bacterial cultures are affected by different solutions. Dilution process commences after a set time of intervals and then the result is mixed with agar. Once the incubation is completed the amount of bacteria is calculated and the number shows the effectiveness of the different solutions of antimicrobial capabilities. The data was documented by using Microsoft office excel 2016 program. Results and conclusions: the weakest of antimicrobial capabilities was the solution, which had PHMB (0,0001 %) - the most intensive decline of bacteria concentration was in the first four hours. The solution with PHMB (0,00015 %) and chamomile had the stronger antimicrobial capabilities (Stpahylococcus epidermidis, Klebsiella pneumoniae and Bacillus cereus bacteria were killed at the end of research). In different time periods most of tested bacteria were killed by the solution with boric acid. The solution with PHMB (0,0002 %) and aloe had a stronger effect and after two hours there was no bacteria. The strongest antimicrobial capabilities were found in the solution, which had H2O2 (3 %) in its' composition - most of the bacteria did not grow after thirty minutes.