Vaisių antimikrobinio aktyvumo nustatymas

Stonkutė, Karolina

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Vaisių antimikrobinio aktyvumo nustatymas

Stonkutė, Karolina

URI: http://dspace.kaunokolegija.lt//handle/123456789/1637

Data: 2019-06-14

Santrauka:

Baigiamajame bakalauro darbe nagrinėjama vaisių antimikrobinis aktyvumas. Išnagrinėta mokslinė ir dalykinė literatūra apie vaisių veikliąsias medžiagas, jų poveikį žmogaus sveikatai, atliktas mikrobiologinis tyrimas, padedantis nustatyti vaisių antimikrobinį aktyvumą. Parengtos rezultatų išvados ir rekomendacijos. Darbą sudaro įvadas, du skyriai: literatūrinė dalis bei empirinė tyrimo metodika ir rezultatai, išvados, rekomendacijos, taip pat literatūros sąrašas ir priedai. Darbo apimtis – 38 psl. teksto be priedų, 6 pav., 2 lent., 55 literatūros šaltiniai. Atskirai pridedami priedai

Description:

One of the most important medical problems is the bacteria and their resistance to antibiotics. Scientists have a great challenge in developing drugs, antibiotics against resistant microorganisms that treat infectious diseases. Studies have shown that flavonoids and other biologically active substances in many fruits have a strong antimicrobial effect, which may be a part of a new drug development process. Aim of the study: to evaluate the antimicrobial effect of the fruit. Objectives of the study: 1. To describe in theory the active substances of fruits and their effects on human health. 2. To determine the antimicrobial effect of the fruits. 3. To compare antimicrobial activity of the fruits when they are just picked up, after a month and after 3 months of storage. On the basis of scientific and professional literature, the main active substances in fruits are various phenolic compounds: most of them are flavonoids. These compounds have antioxidant and antibacterial properties. The study was carried out in Kaunas University of Applied Sciences Faculty of Medicine microbiology laboratory. In the study there were used five species of fruits: apples (Pome), pears (Pyrus), plums (Prunus), actinidias (Actinidia), quinces (Chaenomeles). Fruit is being analyzed just after harvesting as well as after 1 and 3 months of storage. The effects of each fruit were investigated on different 8 microorganisms’ culture. Furthermore, the reliability of the test results was repeated 3 times. A total of 120 samples were tested. Also, the arithmetic mean was obtained from the results. The antimicrobial effect of the fruits was determined by using the method of “wells”. The study results were evaluated by measuring the diameter of the inhibitory zone in millimeters, including the “wells” diameters. The antimicrobial activity was determined using eight standard OXOID No. 605068 cultures of microorganisms. It has been found that in 5 different fruits, quince have the strongest antimicrobial effect. On the other hand, none of the fruits were antimicrobial active against C. albicans. The weakest antimicrobial effect has plum juice. The actinidia juice did not affect the tested microorganisms except S. aureus and P. vulgaris. The study results were compared with fresh fruit, followed by the same fruit after 1 and 3 months of storage. Most of the fresh fruits had slightly stronger antimicrobial properties than fruits after 1 and 3 months of storage.

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